Inhibition of the helicase activity of HCV NTPase/helicase by 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide-5 '-triphosphate (ribavirin-TP).

P Borowski; M Lang; A Niebuhr; A Haag; H Schmitz; J Schulze zur Wiesch; J Choe; M A Siwecka; T Kulikowski

doi:10.18388/abp.2001_3908

Inhibition of the helicase activity of HCV NTPase/helicase by 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide-5 '-triphosphate (ribavirin-TP).

P Borowski Abteilung für Virologie, Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany.;
M Lang
A Niebuhr
A Haag
H Schmitz
J Schulze zur Wiesch
J Choe
M A Siwecka
T Kulikowski

DOI: https://doi.org/10.18388/abp.2001_3908

Abstract

In the presented study the ribavirin-TP--an established inhibitor of the NTPase activity of the superfamily NTPase/helicases II--was investigated as an inhibitor of the unwinding activity of the hepatitis C virus (HCV) NTPase/helicase. The kinetics of the reaction revealed that ribavirin-TP reduces the turnover number of the helicase reaction by a mechanism that does not correspond to that of the inhibition of the NTPase activity. Our results suggest that derivatives of ribavirin-TP with enhanced stability towards hydrolytic attack may be effective inhibitors of the enzyme.

Published

2001-09-30

Issue

Vol. 48 No. 3 (2001)

Section

Articles

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