Multidrug resistance-associated protein--reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides.

W Niewiarowski; E Gendaszewska; G Rebowski; M Wójcik; B Mikołajczyk; W Goss; M Soszyński; G Bartosz

doi:10.18388/abp.2000_3971

Multidrug resistance-associated protein--reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides.

W Niewiarowski Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Lódź.;
E Gendaszewska
G Rebowski
M Wójcik
B Mikołajczyk
W Goss
M Soszyński
G Bartosz

DOI: https://doi.org/10.18388/abp.2000_3971

Abstract

Multidrug resistance-associated protein (MRP1) causes cellular drug resistance in several cancer cell lines. In this paper we show that antisense oligonucleotides decrease MRP1 expression in human leukaemia cells. We investigated biological activity of a series of 12 linear phosphorothioate oligonucleotides, complementary to several regions of MRP1 mRNA. The oligonucleotides were administered to leukaemia HL60/ADR cells overexpressing MRP1 protein. Then, the level of MRP1 mRNA was determined by means of semiquantitative RT-PCR and the protein level by reaction with specific monoclonal antibodies. Some of the investigated antisense oligonucleotides decrease the expression level of the MRP1 protein by 46% and its mRNA level by 76%.

Published

2000-12-31

Issue

Vol. 47 No. 4 (2000)

Section

Articles

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