Partial deglycosylation of alpha subunit modifies sturgeon gonadotropin function.

H Zenkevics; V Vose; I Vosekalne; A Bũcena

doi:10.18388/abp.2000_3999

Partial deglycosylation of alpha subunit modifies sturgeon gonadotropin function.

H Zenkevics Laboratory of Biochemistry, Institute of Biology, Latvian University, Salaspils. zenkevics@email.lubi.edu.lv;
V Vose
I Vosekalne
A Bũcena

DOI: https://doi.org/10.18388/abp.2000_3999

Abstract

Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (alphaGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dg alphaGTH + betaGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed that deglycosylation led to changes in the secondary structure of dg alphaGTH and in the alpha-beta recombinant. The sugar moiety of sturgeon alphaGTH is suggested to play an important role in maintaining the biological function of the hormone dimer molecule.

Published

2000-09-30

Issue

Vol. 47 No. 3 (2000)

Section

Articles

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