Detection of damage-recognition proteins from human lymphocytes.

J Lanuszewska; A Cudak; J Rzeszowska-Wolny; P Widłak

doi:10.18388/abp.2000_4024

Detection of damage-recognition proteins from human lymphocytes.

J Lanuszewska Department of Experimental and Clinical Radiobiology, Center of Oncology, Gliwice, Poland.;
A Cudak
J Rzeszowska-Wolny
P Widłak

DOI: https://doi.org/10.18388/abp.2000_4024

Abstract

Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.

Published

2000-06-30

Issue

Vol. 47 No. 2 (2000)

Section

Articles

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