Heparin- and Zn2+-binding proteins from boar seminal plasma.

D Hołody; J Strzezek

doi:10.18388/abp.1999_4116

Heparin- and Zn2+-binding proteins from boar seminal plasma.

D Hołody Department of Animal Biochemistry, Olsztyn University of Agriculture and Technology, Olsztyn-Kortowo, Poland. dholody@art.olsztyn.pl;
J Strzezek

DOI: https://doi.org/10.18388/abp.1999_4116

Abstract

Low molecular mass, heparin-binding proteins from seminal plasma play an important role in gametes interaction whereas plasmatic Zn2+-binding proteins stabilize chromatin and plasmalemma structures and protect spermatozoa in the female reproductive tract. By means of affinity chromatography the heparin- and Zn2+-binding proteins were isolated from boar seminal plasma and both preparations were analyzed by reverse HPLC. Most of the proteins bound to heparine and Zn2+-ions were classified as spermadhesins. Three fractions binding exclusively Zn2+ were isolated. They differ in amino-acid composition, content of glucosamine and content of protein components revealed by SDS/PAGE.

Published

1999-12-31

Issue

Vol. 46 No. 4 (1999)

Section

Articles

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