Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin.

D Kisiel; A Gindzieński; A Gadek

doi:10.18388/abp.1999_4147

Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin.

D Kisiel Department of General and Organic Chemistry, Institute of Chemistry, Medical Academy of Białystok, Poland.;
A Gindzieński
A Gadek

DOI: https://doi.org/10.18388/abp.1999_4147

Abstract

O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.

Published

1999-09-30

Issue

Vol. 46 No. 3 (1999)

Section

Articles

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