Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene.

M Pietrowska; M Rusin; P Widłak; S V Razin; J Rzeszowska-Wolny

doi:10.18388/abp.1999_4150

Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene.

M Pietrowska Department of Experimental and Clinical Radiobiology, Center of Oncology, Gliwice, Poland.;
M Rusin
P Widłak
S V Razin
J Rzeszowska-Wolny

DOI: https://doi.org/10.18388/abp.1999_4150

Abstract

We have analyzed the DNA fragment localized about 11 to 17.5 kb upstream of the chicken alpha-globin gene domain (the fragment was designed as alpha-0). The nucleotide sequence of its 3.3 kb-long 5' part was established and interactions with nuclear matrix proteins were studied. The DNA region localized about 16 kb upstream of the embryonic pi-globin gene showed high affinity to nuclear matrices in vitro. Two palindromes and a cluster of inverted repeats were co-localized in the same region. The whole 6.6 kb alpha-0 fragment decreased the activity of linked CAT reporter gene when transfected into chicken erythroblastoid cells.

Published

1999-09-30

Issue

Vol. 46 No. 3 (1999)

Section

Articles

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