Nanospray mass spectrometry for identification of peptides. Application of a novel interface.

M T Smoluch; M Sällberg; J Silberring

doi:10.18388/abp.1999_4178

Nanospray mass spectrometry for identification of peptides. Application of a novel interface.

M T Smoluch Faculty of Chemistry, Jagiellonian University, Kraków, Poland.;
M Sällberg
J Silberring

DOI: https://doi.org/10.18388/abp.1999_4178

Abstract

Electrospray ionization mass spectrometry is a powerful tool for identification of biomolecules such as peptides, proteins, oligosaccharides and neurotransmitters. Recent development of the nanospray techniques, applied at ultralow flow-rates, allowed a sensitive analysis of compounds at femto/attomolar level. Here, we present application of a novel nanospray device for the analysis and fragmentation of peptides with high sensitivity on a sector instrument. The lowest applied flow-rate of the mobile phase was maintained at 50 nl/min with a sample load of 90 fmol. Nanospray also provided a complete analysis of 500 nl of the sample for over 10 min, including sequencing of as little as 40 pmol of a substance. Such analysis provides full structural information necessary to identify the molecules.

Published

1999-06-30

Issue

Vol. 46 No. 2 (1999)

Section

Articles

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