Oxidative damage to plant DNA in relation to growth conditions.

K Białkowski; R Oliński

doi:10.18388/abp.1999_4182

Oxidative damage to plant DNA in relation to growth conditions.

K Białkowski Department of Clinical Biochemistry, University School of Medical Sciences, Bydgoszcz, Poland. karolb@monsun.amb.bydgoszcz.pl;
R Oliński

DOI: https://doi.org/10.18388/abp.1999_4182

Abstract

In this study we investigated the level of 8-oxo-2'-deoxyguanosine (8-oxodG) in DNA of Cardamine pratensis plants subjected to different growth conditions trying to answer the question whether factors like light and water accessibility or low temperature may have an impact on the total DNA oxidative damage. The level of this modified nucleoside was determined using HPLC coupled to UV absorbance and electrochemical detection (HPLC-UV-EC). We did not observe any statistically significant differences in 8-oxodG level between DNA of etiolated and light exposed plants as well as between DNA of regularly watered and drought-subjected plants. In contrast, we have shown that chilling (1 degree C for 28 h) brings about the increase of 8-oxodG level in DNA.

Published

1999-03-31

Issue

Vol. 46 No. 1 (1999)

Section

Articles

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