Modulatory effect of divalent metal cations on the phosphotyrosine activity of the frog liver acid phosphatase.

A Szalewicz; B Strzelczyk; A Kubicz

doi:10.18388/abp.1999_4200

Modulatory effect of divalent metal cations on the phosphotyrosine activity of the frog liver acid phosphatase.

A Szalewicz Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland. agata@bf.uni.wroc.pl;
B Strzelczyk
A Kubicz

DOI: https://doi.org/10.18388/abp.1999_4200

Abstract

Frog liver acid phosphatase hydrolyzes phosphotyrosine at acidic pH optimum. Mn2+, Ca2+ and Mg2+ (but not Zn2+) ions modulate this activity by shifting its pH optimum to physiological pH. This effect is not observed when p-nitrophenylphosphate is used as a substrate. Phosphoserine and phosphothreonine are not hydrolyzed under the same conditions.

Published

1999-03-31

Issue

Vol. 46 No. 1 (1999)

Section

Articles

Acta Biochimica Polonica is an OpenAccess quarterly and publishes four issues a year. All contents are distributed under the Creative Commons Attribution-ShareAlike 4.0 International (CC BY 4.0) license. Everybody may use the content following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.