Cloning and sequencing of cDNA encoding the rice methionyl-tRNA synthetase.

M Deniziak; M Mirande; J Barciszewski

doi:10.18388/abp.1998_4205

Cloning and sequencing of cDNA encoding the rice methionyl-tRNA synthetase.

M Deniziak Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Poznań.;
M Mirande
J Barciszewski

DOI: https://doi.org/10.18388/abp.1998_4205

Abstract

Three overlapping clones of cDNA, Mos43, Mos28 and Mos60, coding for methionyl-tRNA synthetase were obtained by screening the Oryza sativa lambda gt11 library. Their nucleotide sequence of 2850 bp was determined. The deduced amino-acid sequence of the isolated clones contains a HLGN and KFSKS motifs, which are conserved for this family of enzymes and have been proposed to be the signature sequences for class I aminoacyl-tRNA synthetases. A comparison of the rice MetRS primary structure with those deposited in EMBL/GenBank points to its high homology to yeast, human and Caenorhabditis elegans MetRSs. Interestingly, a great similarity of its C terminus to endothelial-monocyte-activating polypeptide II (EMAPII) and yeast protein G4p1 was observed.

Published

1998-09-30

Issue

Vol. 45 No. 3 (1998)

Section

Articles

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