Reconstitution of UV-damaged DNA into chromatin using Xenopus oocyte extracts.

P Widłak

doi:10.18388/abp.1998_4252

Reconstitution of UV-damaged DNA into chromatin using Xenopus oocyte extracts.

P Widłak Department of Experimental and Clinical Radiobiology, Institute of Oncology, Gliwice, Poland. widlak@onkol.instonko.gliwice.pl;

DOI: https://doi.org/10.18388/abp.1998_4252

Abstract

Chromatin was reconstituted in vitro using Xenopus oocyte extracts and plasmid DNA containing UV radiation-induced damage. Damaged DNA was assembled into minichromosomes with an efficiency similar to that of control, non-irradiated DNA. Oocyte extracts were competent to carry out DNA repair, which was elicited by nicking damaged templates followed by DNA synthesis during chromatin assembly. Newly synthesized DNA was efficiently reconstituted into nucleosomes.

Published

1998-06-30

Issue

Vol. 45 No. 2 (1998)

Section

Articles

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