Beta-galactosidase in immobilized cells of gherkin Cucumis sativus L.

J Stano; P Nemec; L Bezáková; D Kákoniová; P Kovács; K Neubert; D Liskova; F Andriamainty; K Micieta

doi:10.18388/abp.1998_4255

Beta-galactosidase in immobilized cells of gherkin Cucumis sativus L.

J Stano Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.;
P Nemec
L Bezáková
D Kákoniová
P Kovács
K Neubert
D Liskova
F Andriamainty
K Micieta

DOI: https://doi.org/10.18388/abp.1998_4255

Abstract

Cell suspensions of gherkin (Cucumis sativus L.) were permeabilized by Tween-80, and immobilized by glutaraldehyde. Beta-galactosidase showed pH optimum at 4.9 and temperature optimum at 58 degrees C. The enzyme catalysed hydrolysis was linear for 3 h with 60-68% conversion of the substrate. The cells characterized by high beta-galactosidase activity and stability on long-term storage showed valuable technological properties.

Published

1998-06-30

Issue

Vol. 45 No. 2 (1998)

Section

Articles

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