Age-related profile of beta-N-acetylhexosaminidase glycosylation in rat liver.

A Lityńska; M Przybyło

doi:10.18388/abp.1998_4273

Age-related profile of beta-N-acetylhexosaminidase glycosylation in rat liver.

A Lityńska Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland. LITA@zuk.iz.uj.edu.pl;
M Przybyło

DOI: https://doi.org/10.18388/abp.1998_4273

Abstract

Beta-N-acetylhexosaminidase was prepared from a liver lysosomal fraction obtained from rats between 18 days of gestation (group I) and 72 weeks of age (groups II-VI). A glycan chain analysis was performed after an electrophoresis and blotting, followed by a very sensitive detection system with highly specific digoxigenin-labelled lectins. The presence of high-mannose/hybrid type glycans, as well as their fucosylated forms was shown in all the experimental groups. Complex-type glycans with terminal sialic acid or galactose were present in all the groups except for 1-week-old rats in which only a positive reaction with lectins from Galanthus nivalis and Aleuria aurantia was observed. Thus it may be assumed that age-related changes in the glycosylation pattern occur on the first days after birth.

Published

1998-09-30

Issue

Vol. 45 No. 3 (1998)

Section

Articles

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