The effect of inorganic pyrophosphate on the transport of oleanolic acid monoglycosides into vacuoles isolated from Calendula officinalis leaves.

  • A Szakiel Institute of Biochemistry, Warsaw University, Warszawa, Poland.;
  • W Janiszowska

Abstract

The influence of exogenous inorganic pyrophosphate on the transport of oleanolic acid monoglucoside and monoglucuronide to vacuoles isolated from Calendula officinalis leaf protoplasts was studied. The results indicate that the transport of both monoglycosides is carrier-mediated; however, the transport of the monoglucuronide is passive, and that of the monoglucoside active. The active transport of the monoglucoside is dependent on tonoplast energization created as a result of cooperation of two vacuolar proton pumps: H+-ATPase and H+-PPase.
Published
1998-09-30
Section
Articles