Biochemical and genetic analysis of lambdaW, the newly isolated lambdoid phage.

B Wróbel; S Srutkowska; G Wegrzyn

doi:10.18388/abp.1998_4308

Biochemical and genetic analysis of lambdaW, the newly isolated lambdoid phage.

B Wróbel Department of Molecular Biology, University of Gdańsk, Poland.;
S Srutkowska
G Wegrzyn

DOI: https://doi.org/10.18388/abp.1998_4308

Abstract

Otherwise isogenic Escherichia coli CP78 (relA+) and CP79 (relA-) strains are commonly used in studies on the stringent control, the bacterial response to amino acid starvation. We found that these strains are lysogenic for a phage which is spontaneously induced with a low frequency, producing virions able to infect other E. coli strains. Genetic studies, restriction analysis of the phage DNA genome, and electron microscopy revealed that this phage is very similar to, but not identical with, bacteriophage lambda. We called the newly isolated phage lambdaW, and found that most of CP78/CP79 ancestor strains are lysogenic for this phage.

Published

1998-03-31

Issue

Vol. 45 No. 1 (1998)

Section

Articles

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