Synthesis, biochemical and biological studies on oligonucleotides bearing a lipophilic dimethoxytrityl group.

K Misiura; M Wójcik; A Krakowiak; M Koziołkiewicz; Z Pawłowska; E Pluskota; C S Cierniewski; W J Stec

doi:10.18388/abp.1998_4315

Synthesis, biochemical and biological studies on oligonucleotides bearing a lipophilic dimethoxytrityl group.

K Misiura Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lódź. kmisiura@bio.cbmm.lodz.pl;
M Wójcik
A Krakowiak
M Koziołkiewicz
Z Pawłowska
E Pluskota
C S Cierniewski
W J Stec

DOI: https://doi.org/10.18388/abp.1998_4315

Abstract

Dimethoxytritylphosphono-oligonucleotide conjugates have been prepared. They are totally resistant to nucleases present in human serum and do not affect cleavage of a complementary oligoribonucleotide by RNase H. Conjugates possessing a phosphate backbone gave better antisense inhibition of expression of plasminogen activator inhibitor type-1 within endothelial cells as compared with unconjugated oligonucleotides.

Published

1998-03-31

Issue

Vol. 45 No. 1 (1998)

Section

Articles

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