Dolichyl sulphate and H-phosphonate: enzymatic reactions with activated sugars.

O V Sizova; S D Maltsev; V N Shibaev; W J Jankowski; T Chojnacki

doi:10.18388/abp.1998_4359

Dolichyl sulphate and H-phosphonate: enzymatic reactions with activated sugars.

O V Sizova N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow. siz@ioc.ac.ru;
S D Maltsev
V N Shibaev
W J Jankowski
T Chojnacki

DOI: https://doi.org/10.18388/abp.1998_4359

Abstract

Two phosphate-modified analogues of dolichyl phosphate were evaluated as substrates or inhibitors of the reactions catalyzed by mammalian microsomal enzymes. Dolichyl H-phosphonate could serve as an efficient acceptor for mannosyl and glucosyl transfer. The reaction products were chromatographically different from those formed from dolichyl phosphate. Lower activity of the H-phosphonate was observed for the reaction of N-acetylglucosaminyl phosphate transfer from UDP-GlcNAc. Dolichyl sulphate was shown not to serve as a substrate for the transfer of mannosyl (from GDP-Man), glucosyl (from UDP-Glc) or N-acetylglucosaminyl phosphate (from UDP-GlcNAc) residues in the presence of rat liver microsomes. Weak inhibitory properties of this analogue were demonstrated.

Published

1998-12-31

Issue

Vol. 45 No. 4 (1998)

Section

Articles

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