Chemical modification of lysine and arginine residues of bovine heart 2-oxoglutarate dehydrogenase: effect on the enzyme activity and regulation.

S A Ostrovtsova

doi:10.18388/abp.1998_4360

Chemical modification of lysine and arginine residues of bovine heart 2-oxoglutarate dehydrogenase: effect on the enzyme activity and regulation.

S A Ostrovtsova Institute of Biochemistry, National Academy of Sciences of Belarus, Grodno.;

DOI: https://doi.org/10.18388/abp.1998_4360

Abstract

Chemical modification of arginine and lysine residues of bovine heart 2-oxoglutarate dehydrogenase with phenylglyoxal and pyridoxal 5'-phosphate inactivated the enzyme, indicating the importance of these residues for the catalysis. Inactivation caused by pyridoxal 5'-phosphate was prevented in the presence of thiamine pyrophosphate and Mg2+ allowing the assumption that lysine residues participate in binding of the cofactor.

Published

1998-12-31

Issue

Vol. 45 No. 4 (1998)

Section

Articles

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