Molecular characterization and symbiotic importance of prsD gene of Rhizobium leguminosarum bv. trifolii TA1.

A Mazur; J Wielbo; J Król; J Kopcińska; B Lotocka; W Golinowski; A Skorupska

doi:10.18388/abp.1998_4364

Molecular characterization and symbiotic importance of prsD gene of Rhizobium leguminosarum bv. trifolii TA1.

A Mazur Department of General Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.;
J Wielbo
J Król
J Kopcińska
B Lotocka
W Golinowski
A Skorupska

DOI: https://doi.org/10.18388/abp.1998_4364

Abstract

The prsD, prsE and orf3 genes of Rhizobium leguminosarum bv. trifolii strain TA1 encode the proteins which are significantly related to the family of bacterial ABC transporters type I secretion systems. The prsD:Km(r) mutant of strain TA1 induced non-nitrogen-fixing nodules on Trifolium pratense. Microscopic analysis of the nodules induced by prsD mutant did not reveal major abberations in the bacteroid appearance. The exopolysaccharide of prsD mutant was produced in increased amount and its level of polymerization was changed. SDS/PAGE of the proteins from the culture supernatants showed a lack of the 47-kDa protein in the culture of prsD mutant. Thus, PrsD may play a role in the export of this protein.

Published

1998-12-31

Issue

Vol. 45 No. 4 (1998)

Section

Articles

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