Crystallization and preliminary crystallographic studies of a new crystal form of papain from Carica papaya.

M Kozak; E Kozian; Z Grzonka; M Jaskólski

doi:10.18388/abp.1997_4408

Crystallization and preliminary crystallographic studies of a new crystal form of papain from Carica papaya.

M Kozak Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznań, Poland.;
E Kozian
Z Grzonka
M Jaskólski

DOI: https://doi.org/10.18388/abp.1997_4408

Abstract

A new crystal form of papain from the latex of Carica papaya, complexed with an inhibitor (Z-Arg-Leu-Val-Gly-CHN2) was obtained by the vapor-diffusion method using a methanol/ethanol mixture as a precipitant. The slat-like crystals are monoclinic, space group P2(1), with unit cell parameters a = 42.6 A, b = 49.8 A, c = 50.5 A, A = 111.9 degrees, and contain one molecule in the asymmetric unit. The crystals are stable in the X-ray beam and diffract beyond 1.8 A. A molecular model has been placed in the unit cell by molecular replacement.

Published

1997-09-30

Issue

Vol. 44 No. 3 (1997)

Section

Articles

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