Characterization of the oligosaccharide component of arylsulfatase B from rat liver.

M Przybyło; A Lityńska

doi:10.18388/abp.1997_4412

Characterization of the oligosaccharide component of arylsulfatase B from rat liver.

M Przybyło Department of Animal Physiology, Jagiellonian University, Cracow, Poland. kloc@zuk.iz.uj.edu.pl;
A Lityńska

DOI: https://doi.org/10.18388/abp.1997_4412

Abstract

A glycan chain analysis of the total oligosaccharide pool derived from rat liver arylsulfatase B was carried out by. P4 Gel Permeation Chromatography and sequential exoglycosidase digestion. It was found that 71% of rat liver arylsulfatase B oligosaccharides were sialylated. The relative contribution of particular structures in the total glycan pool was as follows: sialylated biantennary complex type glycans with terminal galactose--65%, high-mannose type glycans--15%, biantennary complex type glycans with core fucose and terminal N-acetylglucosamine--5%, O-linked oligosaccharides--3.5%.

Published

1997-06-30

Issue

Vol. 44 No. 2 (1997)

Section

Articles

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