The N-acetylgalactosamine and lactosamine specific lectin from Iris hybrida leaves.

M Ferens-Sieczkowska; M Orczyk-Pawiłowicz; B Morawiecka

doi:10.18388/abp.1997_4425

The N-acetylgalactosamine and lactosamine specific lectin from Iris hybrida leaves.

M Ferens-Sieczkowska Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland.;
M Orczyk-Pawiłowicz
B Morawiecka

DOI: https://doi.org/10.18388/abp.1997_4425

Abstract

The lectin isolated from the leaves of Iris hybrida binds specifically N-acetyl-galactosamine and lactose. Its molecule consists of two identical subunits bound by disulfide bonds. The lectin is a glycoprotein containing about 12% of sugars. It binds asialoglycoproteins containing complex type sugar chains. The binding is reduced by half at the concentration of 0.15 to 0.40 mM of the galactose containing disaccharides irrespectively to a type of galactose isomer. This indicates rather broad specificity of I. hybrida leaf lectin.

Published

1997-06-30

Issue

Vol. 44 No. 2 (1997)

Section

Articles

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