Studies on the translation mechanism of subgenomic RNA of potato leafroll virus.

M Juszczuk; W Zagórski-Ostoja; D M Hulanicka

doi:10.18388/abp.1997_4441

Studies on the translation mechanism of subgenomic RNA of potato leafroll virus.

M Juszczuk Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.;
W Zagórski-Ostoja
D M Hulanicka

DOI: https://doi.org/10.18388/abp.1997_4441

Abstract

The expression of open reading frames located on the subgenomic RNA (sgRNA) has been studied in an in vitro transcription and translation system. The obtained results indicate: a) translation of sgRNA occurs according to the scanning model since the insertion of palindrome (delta G0 = -61 kcal/mol) prevents the initiation of translation; b) ORF6 is translated by suppression of the stop codon separating ORF4 from ORF6 and the presence of suppressor tRNA is necessary for the readthrough; c) the presence of leader sequence of sgRNA (212 nucleotides) decreases the translation efficiency of ORFs located downstream and it affects the ratio of products of ORF4 and ORF5; d) 3'UTR does not influence on an expression of genes located on the sgRNA.

Published

1997-03-31

Issue

Vol. 44 No. 1 (1997)

Section

Articles

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