Autonomous replication of a wheat DNA sequence in isolated wheat nuclei.

B Szurmak; J Buchowicz

doi:10.18388/abp.1997_4442

Autonomous replication of a wheat DNA sequence in isolated wheat nuclei.

B Szurmak Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.;
J Buchowicz

DOI: https://doi.org/10.18388/abp.1997_4442

Abstract

A fragment of wheat nuclear DNA was shown to be able to replicate autonomously in wheat nuclei. The fragment was 637-bp long and contained telomeric repeats at both termini. Its replication was manifested by the appearance of a radioactive reaction product of the same approximate size. Further analyses showed that the linear, double-stranded reaction product was labelled along its entire length and contained the same number of similarly located HaeIII sites as did the fragment tested. Prokaryotic DNA remained unlabelled under the same assay conditions.

Published

1997-03-31

Issue

Vol. 44 No. 1 (1997)

Section

Articles

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