Activity of mu- and m-calpain in regenerating fast and slow twitch skeletal muscles.

J Moraczewski; E Piekarska; M Zimowska; M Sobolewska

doi:10.18388/abp.1996_4466

Activity of mu- and m-calpain in regenerating fast and slow twitch skeletal muscles.

J Moraczewski Department of Cytology, Institute of Zoology, University of Warsaw, Poland. jmlabo@plearn.edu.pl;
E Piekarska
M Zimowska
M Sobolewska

DOI: https://doi.org/10.18388/abp.1996_4466

Abstract

Calpains--non-lysosomal intracellular calcium-activated neutral proteinases, form a family consisting of several distinct members. Two of the isoenzymes: mu (calpain I) and m (calpain II) responded differently to the injury during complete regeneration of Extensor digitorum longus (EDL) muscle and partial regeneration of Soleus muscle. In the crushed EDL the level of m-calpain on the 3rd and 7th day of regeneration was higher than in non-operated muscles, whereas the activity of this calpain in injured Soleus decreased. The level of mu-calpain in EDL oscillated irregularly during regeneration whereas in Soleus of both injured and contralateral muscles its level rapidly rose. Our results support the hypothesis that m-calpain is involved in the process of fusion of myogenic cells whereas mu-calpain plays a significant but indirect role in muscle regeneration.

Published

1996-12-31

Issue

Vol. 43 No. 4 (1996)

Section

Articles

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