The use of sequential affinity chromatography for separation of human neutrophil elastase, cathepsin G and azurocidin.

W Watorek; A Polanowski; T Wilusz

doi:10.18388/abp.1996_4483

The use of sequential affinity chromatography for separation of human neutrophil elastase, cathepsin G and azurocidin.

W Watorek Institute of Biochemistry, Wrocław University, Poland.;
A Polanowski
T Wilusz

DOI: https://doi.org/10.18388/abp.1996_4483

Abstract

Elastase, cathepsin G and azurocidin from human neutrophils are key components of body inflammatory defense. Perturbations in regulation of their activities lead to many serious pathological states. The paper describes a simple, fast and efficient method of joint purification of these proteins with the use of sequential affinity chromatography on squash trypsin inhibitor (CMTI I) and bovine pancreatic trypsin inhibitor (BPTI).

Published

1996-09-30

Issue

Vol. 43 No. 3 (1996)

Section

Articles

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