3' Noncoding sequences of the CTA 1 gene enhance expression of the recombinant serine protease inhibitor, CPTI II, in Saccharomyces cerevisiae.

M Stankiewicz; B Rempoła; M Fikus

doi:10.18388/abp.1996_4487

3' Noncoding sequences of the CTA 1 gene enhance expression of the recombinant serine protease inhibitor, CPTI II, in Saccharomyces cerevisiae.

M Stankiewicz Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.;
B Rempoła
M Fikus

DOI: https://doi.org/10.18388/abp.1996_4487

Abstract

Expression of the gene coding for the recombinant trypsin inhibitor, CPTI II, was enhanced tenfold when yeast transcription terminating sequences were added to the expression cassette of the pJK6 yeast vector. The yield was further increased about 20% in the BJ5464 yeast strain, defective in vacuolar proteases.

Published

1996-09-30

Issue

Vol. 43 No. 3 (1996)

Section

Articles

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