Purification of IHF-like protein from gram-negative bacteria in one chromatographic step.

B Krawczyk; J Kur

doi:10.18388/abp.1996_4507

Purification of IHF-like protein from gram-negative bacteria in one chromatographic step.

B Krawczyk Department of Microbiology, Technical University of Gdańsk, Poland.;
J Kur

DOI: https://doi.org/10.18388/abp.1996_4507

Abstract

We describe a fast and very efficient method of purification which yields highly purified integration host factor-like proteins in one chromatographic step. IHF-like proteins from Acinetobacter junii or Proteus vulgaris are each an alpha beta heterodimer (subunits of 10 and 11 kDa) similar to the IHF of Escherichia coli when analyzed by polyacrylamide gel electrophoresis. The purified IHF are able to bind to the same ihf sites as IHF of E. coli. The results presented confirm that IHF is conserved during evolution in gram-negative bacteria.

Published

1996-06-30

Issue

Vol. 43 No. 2 (1996)

Section

Articles

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