Protective effect of pantothenic acid and related compounds against permeabilization of Ehrlich ascites tumour cells by digitonin.

V S Slyshenkov; M Rakowska; L Wojtczak

doi:10.18388/abp.1996_4512

Protective effect of pantothenic acid and related compounds against permeabilization of Ehrlich ascites tumour cells by digitonin.

V S Slyshenkov M. Nencki Institute of Experimental Biology, Warsaw, Poland.;
M Rakowska
L Wojtczak

DOI: https://doi.org/10.18388/abp.1996_4512

Abstract

Preincubation of Ehrlich ascites tumour cells with millimolar concentrations of pantothenic acid, pantothenol or pantethine, but not with homopantothenic acid, at 22 degrees C or 32 degrees C, but not at 0 degrees C, makes the plasma membrane more resistant to the damaging effect of submillimolar concentrations of digitonin. It is proposed that this increased resistance is due to the increased rate of cholesterol biosynthesis. In fact, incorporation of [14C]acetate into cholesterol is by 45% increased in the cells preincubated with pantothenic acid; this probably reflects elevation of the content of CoA in such cells [Slyshenkov, V.S., Rakowska, M., Moiseenok, A.G. & Wojtczak, L. (1995) Free Radical Biol. Med. 19, 767-772].

Published

1996-06-30

Issue

Vol. 43 No. 2 (1996)

Section

Articles

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