Nucleotide probes of DNA polymerases.

G E Wright

doi:10.18388/abp.1996_4522

Nucleotide probes of DNA polymerases.

G E Wright Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester 01655, USA.;

DOI: https://doi.org/10.18388/abp.1996_4522

Abstract

The modified nucleotides, N2-(p-n-butylphenyl)dGTP and 2-(p-n-butylanilino) dATP and related compounds have been developed as inhibitor-probes of B family DNA polymerases. Synthetic approaches to these compounds are summarized. The nucleotides are potent, non-substrate inhibitors of DNA polymerase a. In contrast, they inhibit other members of the family with less potency but act as substrates for these enzymes. Modelling of the inhibitor: enzyme binding mechanism has been done based on the known structure of E. coli DNA polymerase I, and site-directed mutagenesis experiments to evaluate this mechanism are proposed.

Published

1996-03-31

Issue

Vol. 43 No. 1 (1996)

Section

Articles

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