Miscoding properties of isoguanine (2-oxoadenine) studied in an AMV reverse transcriptase in vitro system.

A M Bukowska; J T Kuśmierek

doi:10.18388/abp.1996_4558

Miscoding properties of isoguanine (2-oxoadenine) studied in an AMV reverse transcriptase in vitro system.

A M Bukowska Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.;
J T Kuśmierek

DOI: https://doi.org/10.18388/abp.1996_4558

Abstract

We have found that isoguanine (iG) can pair with thymine (iG.T) and the non-natural base, 5-methylisocytosine (iG.iCM) during template directed synthesis catalyzed by AMV reverse transcriptase. The ratio of these pairings is about 1:10, irrespectively which of the templates, poly(C,iG) or poly(I,iG) is used. This ratio corresponds to the ratio of 2-OH and 2-keto tautomers in monomer in aqueous solution and apparently it is not influenced by the template context. Our results indicate also that formation of the reverse transcriptase catalyzed base pairs between iG and A, G or C can occur only at a low frequency, comparable to the frequency, of mismatches of.(ABSTRACT TRUNCATED)

Published

1996-03-31

Issue

Vol. 43 No. 1 (1996)

Section

Articles

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