Expression of small synthetic genes coding for hEGF, human epidermal growth factor, and CPTI II, serine proteinase inhibitor from Cucurbitacea, cloned in a novel expression/secretion vector in Saccharomyces cerevisiae.

J Topczewska; B Rempoła; M Fikus

doi:10.18388/abp.1996_4559

Expression of small synthetic genes coding for hEGF, human epidermal growth factor, and CPTI II, serine proteinase inhibitor from Cucurbitacea, cloned in a novel expression/secretion vector in Saccharomyces cerevisiae.

J Topczewska Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.;
B Rempoła
M Fikus

DOI: https://doi.org/10.18388/abp.1996_4559

Abstract

Efficient synthesis of two small eukaryotic polypeptides of human and plant origin was carried out using a novel expression/secretion yeast vector, pYET. The yield was optimized in respect of the yeast strain, expression cassette construction, promoter regulation and culture conditions. Both cloned genes code for biotechnologically important proteins: human epidermal growth factor and a serine proteinase inhibitor from Cucurbitacea.

Published

1996-03-31

Issue

Vol. 43 No. 1 (1996)

Section

Articles

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