Effect of Ca2+ on the activity of mitochondrial NADP-specific isocitrate dehydrogenase from rabbit adrenals.

E Strumiło

doi:10.18388/abp.1995_4628

Effect of Ca2+ on the activity of mitochondrial NADP-specific isocitrate dehydrogenase from rabbit adrenals.

E Strumiło Laboratory of Endocrine Glands Biochemistry, Academy of Sciences of Belarus, Grodno, Belarus.;

DOI: https://doi.org/10.18388/abp.1995_4628

Abstract

Mitochondrial NADP-specific isocitrate dehydrogenase (ICDH) partially purified from rabbit adrenals is sensitive to low concentrations of Ca2+. In the absence of Ca2+ (addition of 1 mM EGTA) the Km of ICDH for DL-isocitrate was 143 microM, and in the presence of about 10 microM Ca2+ (EGTA-Ca2+ buffer) it was decreased to 55 microM. The Km value of ICDH for NADP was similar in the absence (4.4 microM) and in the presence (5.0 microM) of Ca2+. The Km of mitochondrial NADP-ICDH for DL-isocitrate was higher than that of cytoplasmic NADP-ICDH from the same source.

Published

1995-09-30

Issue

Vol. 42 No. 3 (1995)

Section

Articles

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