Some regulatory properties of the 2-oxoglutarate dehydrogenase complex from European bison heart.

J Markiewicz; S A Strumiło

doi:10.18388/abp.1995_4631

Some regulatory properties of the 2-oxoglutarate dehydrogenase complex from European bison heart.

J Markiewicz Department of Biochemistry, University of Warsaw, Białystok, Poland.;
S A Strumiło

DOI: https://doi.org/10.18388/abp.1995_4631

Abstract

Basic regulatory properties of the 2-oxoglutarate dehydrogenase complex (OGDC) isolated and purified from the heart muscle of European bison (Bison bonasus) were studied. Kinetic studies have shown that in the absence of phosphate ions OGDC exhibits kinetic attributes of negative cooperativity with respect to 2-oxoglutarate. ADP and phosphate lower S0.5 value of OGDC for 2-oxoglutarate without changing the maximum reaction rate. NADH inhibits OGDC versus both 2-oxoglutarate and NAD+. Moreover, bison heart OGDC shows negative kinetic cooperativity for NAD+ and positive kinetic cooperativity for CoA at low CoA concentrations. The latter property has not been observed in earlier studies on OGDC from bovine and pig heart and other tissues of these animals.

Published

1995-09-30

Issue

Vol. 42 No. 3 (1995)

Section

Articles

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