Expression analysis of a Cucurbita cDNA encoding endonuclease.

J Szopa

doi:10.18388/abp.1995_4643

Expression analysis of a Cucurbita cDNA encoding endonuclease.

J Szopa Institute of Biochemistry, Wrocław University, Poland.;

DOI: https://doi.org/10.18388/abp.1995_4643

Abstract

The nuclear matrices of plant cell nuclei display intrinsic nuclease activity which consists in nicking supercoiled DNA. A cDNA encoding a 32 kDa endonuclease has been cloned and sequenced. The nucleotide and deduced amino-acid sequences show high homology to known 14-3-3 protein sequences from other sources. The amino-acid sequence shows agreement with consensus sequences for potential phosphorylation by protein kinase A and C and for calcium, lipid and membrane-binding sites. The nucleotide-binding site is also present within the conserved part of the sequence. By Northern blot analysis, the differential expression of the corresponding mRNA was detected; it was the strongest in sink tissues. The endonuclease activity found on DNA-polyacrylamide gel electrophoresis coincided with mRNA content and was the highest in tuber.

Published

1995-06-30

Issue

Vol. 42 No. 2 (1995)

Section

Articles

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