Synthesis of 5-IASA-UDP-GlcNAc and its use for the photoaffinity labeling of a novel UDP-GlcNAc pyrophosphorylase.

H Szumiło; T Szumiło; A D Elbein

doi:10.18388/abp.1995_4668

Synthesis of 5-IASA-UDP-GlcNAc and its use for the photoaffinity labeling of a novel UDP-GlcNAc pyrophosphorylase.

H Szumiło Department of Inorganic and Analytical Chemistry, Medical School, Lublin, Poland.;
T Szumiło
A D Elbein

DOI: https://doi.org/10.18388/abp.1995_4668

Abstract

Photoreactivable 5-[3-(p-iodoazidosalicylamide)allyl]-UDP-GlcNAc (5-IASA-UDP-GlcNAc) was synthesized by a four-step procedure and used for photoaffinity labeling of UDP-GlcNAc-dependent enzymes. Upon iodination with 125I, the compound was successfully applied to probe a purified UDP-GlcNAc pyrophosphorylase from pig liver. The enzyme was photoinactivated by the probe in the concentration-dependent manner, and was protected by UDP-GlcNAc and, to a lesser extent, by UTP and UDP-GlcCOOH.

Published

1995-03-31

Issue

Vol. 42 No. 1 (1995)

Section

Articles

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