Expression of the plum pox coat protein gene in transgenic Nicotiana tabacum plants.

K J Wypijewski; W G Musiaø; L Misztal; B Pater; T Malinowski; J Augustyniak

doi:10.18388/abp.1995_4674

Expression of the plum pox coat protein gene in transgenic Nicotiana tabacum plants.

K J Wypijewski A. Mickiewicz University, Department of Biopolymer Biochemistry, Poznań, Poland.;
W G Musiaø
L Misztal
B Pater
T Malinowski
J Augustyniak

DOI: https://doi.org/10.18388/abp.1995_4674

Abstract

Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCM1). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.

Published

1995-03-31

Issue

Vol. 42 No. 1 (1995)

Section

Articles

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