Synthesis, cloning and expression in Escherichia coli of the gene coding for the trypsin inhibitor from Cucurbita pepo.

B Rempoøa; T Wilusz; W Markiewicz; M Fikus

doi:10.18388/abp.1995_4676

Synthesis, cloning and expression in Escherichia coli of the gene coding for the trypsin inhibitor from Cucurbita pepo.

B Rempoøa Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Warsaw.;
T Wilusz
W Markiewicz
M Fikus

DOI: https://doi.org/10.18388/abp.1995_4676

Abstract

A chemically synthesized gene coding for the serine proteinase inhibitor CPTI II was cloned in E. coli and its expression was investigated in cytoplasmic and secretion systems. Under all conditions investigated the biologically active form of the inhibitor was found only in the latter system, although the yield was rather low.

Published

1995-03-31

Issue

Vol. 42 No. 1 (1995)

Section

Articles

Acta Biochimica Polonica is an OpenAccess quarterly and publishes four issues a year. All contents are distributed under the Creative Commons Attribution-ShareAlike 4.0 International (CC BY 4.0) license. Everybody may use the content following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.