DNA damage and repair in normal and neoplastic cells treated with adriamycin.

E L Anuszewska; B Gruber

doi:10.18388/abp.1994_4682

DNA damage and repair in normal and neoplastic cells treated with adriamycin.

E L Anuszewska Drug Institute, Warsaw, Poland.;
B Gruber

DOI: https://doi.org/10.18388/abp.1994_4682

Abstract

Adriamycin (ADR), a common antineoplastic drug, was used to study DNA repair synthesis, cell cytotoxicity and DNA single strand breaks in normal human fibroblasts--CLV98 and human melanoma cells--ME18. No repair synthesis was observed in ME18 and CLV98 cells exposed to adriamycin in concentrations up to 10(-5) M. ME18 cells were less sensitive to ADR treatment than CLV98 cells. Adriamycin-induced DNA single strand breaks (at ADR concentration: 1 microgram/ml) were incompletely repaired in ME18 cells and unrepaired in CLV98 cells within 24 h after drug removal. Within 48 h strand breaks were completely repaired in both kinds of cells. No repair of single strand breaks was observed in ME18 and CLV98 cells after drug treatment in the concentration of 5 micrograms/ml.

Published

1994-12-31

Issue

Vol. 41 No. 4 (1994)

Section

Articles

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