Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).

M Wełnicki; C Zekanowski; W Zagórski

doi:10.18388/abp.1994_4702

Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).

M Wełnicki Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.;
C Zekanowski
W Zagórski

DOI: https://doi.org/10.18388/abp.1994_4702

Abstract

A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT probe. This makes p3POT probe an useful tool for the routine testing, and selection of virus-free potatoes.

Published

1994-12-31

Issue

Vol. 41 No. 4 (1994)

Section

Articles

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