Nucleotide sequence of the coat protein gene of the Skierniewice isolate of plum pox virus (PPV).

K Wypijewski; T Malinowski; W Musiał; J Augustyniak

doi:10.18388/abp.1994_4778

Nucleotide sequence of the coat protein gene of the Skierniewice isolate of plum pox virus (PPV).

K Wypijewski Department of Biopolymer Biochemistry, A. Mickiewicz University, Poznań, Poland.;
T Malinowski
W Musiał
J Augustyniak

DOI: https://doi.org/10.18388/abp.1994_4778

Abstract

The coat protein (CP) gene of the Skierniewice isolate of plum pox virus (PPV-S) has been amplified using the reverse transcription--polymerase chain reaction (RT-PCR), cloned and sequenced. The nucleotide sequence of the gene and the deduced amino-acid sequence of PPV-S CP were compared with those of other PPV strains. The nucleotide sequence showed very high homology to most of the published sequences. The motif: Asp-Ala-Gly (DAG), important for the aphid transmissibility, was present in the amino-acid sequence. Our isolate did not react in ELISA with monoclonal antibodies MAb06 supposed to be specific for PPV-D.

Published

1994-03-31

Issue

Vol. 41 No. 1 (1994)

Section

Articles

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