Z-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG.

E Biała; J Milecki; A Kowalewski; M Popenda; W Z Antkowiak; R W Adamiak

doi:10.18388/abp.1993_4793

Z-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG.

E Biała Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.;
J Milecki
A Kowalewski
M Popenda
W Z Antkowiak
R W Adamiak

DOI: https://doi.org/10.18388/abp.1993_4793

Abstract

Chemical synthesis of 2'-O-[13C]methyl-rCGCGCG and 5-methyl-rCGCGCG using support-aided phosphoramidite method is presented. 2'-O-Methyl guanosine derivative was separated from its 3'-O-methyl counterpart using transient 5',3'-O-silylation with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane (Markiewicz reagent). The hexamers were obtained in a purity suitable for NMR studies.

Published

1993-12-31

Issue

Vol. 40 No. 4 (1993)

Section

Articles

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