In silico characterization of linoleic acid biotransformation to rumenic acid in food derived Lactobacillus plantarum YW11

Tariq  Aziz; Abid Sarwar; Muhammad Fahim; Sam Al Dalali; Zia  Ud Din; Jalal Ud Din; Zou Xin; Zhang Jian; Taicia Pacheco Fill; Yang Zhennai

doi:10.18388/abp.2020_5095

Tariq Aziz Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology https://orcid.org/0000-0002-4564-5756
Abid Sarwar Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing
Muhammad Fahim Centre for Omic Sciences, Islamia College Peshawar, KP, Pakistan 25120
Sam Al Dalali Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing https://orcid.org/0000-0002-2173-1916
Zia Ud Din Department of Chemistry Universidade Federeal de Sao Carlos, SP, Brazil
Jalal Ud Din Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing
Zou Xin Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing
Zhang Jian Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing
Taicia Pacheco Fill Institute of Chemistry Universidade Estadual de Campinas, SP, Brazil
Yang Zhennai Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing

DOI: https://doi.org/10.18388/abp.2020_5095

Abstract

Lactobacillus plantarum YW11 capability to convert linoleic acid into conjugated linoleic acid and other metabolites was studied in a dose-dependent manner by supplementing LA at different concentrations. L. plantarum YW11 displayed a uniform distinctive growth curve of CLA and other metabolites at concentrations of LA ranging from 1% (w/v) to 10% (w/v), with slightly increased growth at higher LA concentrations. The biotransformation capability of L. plantarum YW11 evaluated by GC-MS revealed a total of one CLA isomer, i.e. 9-cis,11-trans-octadecadienoic acid, also known as the rumenic acid (RA), one linoleic acid isomer (linoelaidic acid), and LA metabolites: (E)-9-octadecenoic acid ethyl ester, trans, trans-9,12-octadecadienoic acid, propyl ester and stearic acid. All the metabolites of linoleic acid were produced from 1 to 10% LA supplemented MRS media, while surprisingly the only conjugated linoleic acid compound was produced at 10% LA. To assess the presence of putative enzymes, responsible for conversion of LA into CLA, in silico characterization was carried out. The in silico characterization revealed presence of four enzymes (10-linoleic acid hydratase, linoleate isomerase, acetoacetate decarboxylase and dehydrogenase) that may be involved in the production of CLA (rumenic acid) and LA isomers. The biotransformation ability of L. plantarum YW11 to convert LA into RA has great prospects for biotechnological and industrial implications that could be exploited in the future scale-up experiments.