Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.

L Bicka; J Kuźmak; B Kozaczyńska; A Plucienniczak; A Skorupska

doi:10.18388/abp.2001_5130

Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.

L Bicka Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland.;
J Kuźmak
B Kozaczyńska
A Plucienniczak
A Skorupska

DOI: https://doi.org/10.18388/abp.2001_5130

Abstract

The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.

Published

2001-03-31

Issue

Vol. 48 No. 1 (2001)

Section

Articles

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