Selected CDKN2A and MDM2 polymorphisms in oral cavity cancer

Jadwiga Gaździcka; Karolina Gołąbek; Joanna Katarzyna Strzelczyk; Paweł Kiczmer; Katarzyna Miśkiewicz-Orczyk; Łukasz Krakowczyk; Zofia Ostrowska; Maciej Misiołek

doi:10.18388/abp.2020_5195

Jadwiga Gaździcka Medical University of Silesia https://orcid.org/0000-0002-3335-2403
Karolina Gołąbek Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Zabrze, Poland https://orcid.org/0000-0002-0042-5856
Joanna Katarzyna Strzelczyk Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Zabrze, Poland https://orcid.org/0000-0002-3686-5685
Paweł Kiczmer Department of Pathomorphology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Zabrze, Poland https://orcid.org/0000-0001-9060-0154
Katarzyna Miśkiewicz-Orczyk Department of Otorhinolaryngology and Oncological Laryngology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland https://orcid.org/0000-0001-8088-3437
Łukasz Krakowczyk Clinic of Oncological and Reconstructive Surgery, Maria Sklodowska–Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland https://orcid.org/0000-0002-6096-5651
Zofia Ostrowska Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Zabrze, Poland https://orcid.org/0000-0002-4301-2429
Maciej Misiołek Department of Otorhinolaryngology and Oncological Laryngology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland https://orcid.org/0000-0002-8476-9153

DOI: https://doi.org/10.18388/abp.2020_5195

Abstract

The head and neck squamous cell carcinoma (HNSCC) is an aggressive human malignancy diagnosed in more than 800 000 new cases worldwide, and mostly arises in the oral cavity, oropharynx, larynx, hypopharynx, and nasopharynx. The study presented here aimed to determine a possible association of rs11515 and rs3088440 gene polymorphisms in the CDKN2A gene (cyclin dependent kinase inhibitor 2A), as well as rs769412 and rs937283 in the MDM2 gene (murine double minute 2), with oral cavity cancer in a sample of Polish population; CDKN2A is crucial in regulating the cell cycle while MDM2 is an oncogene involved in regulating cell proliferation and apoptosis. The study included 95 primary tumor samples following surgical resection from patients, and the control group consisted of 100 healthy individuals. DNA samples were genotyped by employing the 5’ nuclease assay for allelic discrimination using TaqMan SNP Genotyping Assays (Applied Biosystems, USA). There was no significant association between any of the polymorphism (rs11515, rs3088440, rs769412 and rs937283) and the oral cavity cancer risk. We found that the AA homozygotes for rs3088440 were significantly more frequent in the control group (OR=0.046, p<0.0001). In addition, the GG genotype of rs769412 was not found in any group. We found no influence of the examined genotypes on clinicopathological parameters, such as T, N and grading values in patients with oral cavity cancer. The results of this study indicate that none of the investigated polymorphisms were associated with the risk of oral cavity cancer in the examined sample of the Polish population.