Cis-2 and trans-2-eisocenoic fatty acids are novel inhibitors for Mycobacterium tuberculosis Protein tyrosine phosphatase A

  • Lalu Rudyat Telly Savalas Department of Biology Education, Faculty of Teacher Training and Education, University of Mataram
  • Baiq Repika Nurul Furqon Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Mataram https://orcid.org/0000-0001-7219-1780
  • Dina Asnawati Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Mataram https://orcid.org/0000-0001-9135-0767
  • Jannatin 'Ardhuha Department of Physics Education, Faculty of Teacher Training and Education, University of Mataram https://orcid.org/0000-0003-1982-263X
  • Prapti Sedijani Department of Biology Education, Faculty of Teacher Training and Education, University of Mataram
  • Saprizal Hadisaputra Department of Chemistry Education, Faculty of Teacher Training and Education, University of Mataram https://orcid.org/0000-0002-0386-4571
  • Baiq Nila Sari Ningsih Department of Chemistry, Faculty of Science, Prince of Songkhla University, Thailand
  • Jufrizal Syahri Department of Chemistry, Universitas Muhammadiyah Riau, Indonesia https://orcid.org/0000-0002-8918-4779
DOI: https://doi.org/10.18388/abp.2020_5201

Abstract

Small protein tyrosine phosphatase (PtpA) of Mycobacterium tuberculosis is attributed to the development of latent tuberculosis infection, and hence bocomes an interesting target for drug development. In this communication, inhibition of PtpA by naturally occurring fatty acids cis-2 and trans-2-eicosenoic acid is investigated. Mtb PtpA was heterologously expressed in Escherichia coli, and the activity of PtpA was inhibited by cis-2 and trans-2 eicosenoic fatty acids. Both compunds showed strong inhibition of PtpA activity with IC50 at low micromolar concentration. As comparison, trans-11-eicosenoic acid only slightly inhibit PtpA. In silico analysis confirmed the inhibition of PtpB by cis-2-eicosenoic acid by formation of several hydrogen bonds. These findings show that cis-2 and trans-2 eicosenoic fatty acids are potential candidates for latent tuberculosis inhibitors.

Published
2020-06-18
Issue
Vol. 67 No. 2 (2020)
Section
Articles

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