Antioxidative action of polyamines in protection of phospholipid membranes exposed to ozone stress

Elżbieta Rudolphi-Szydło; Maria Filek; Barbara Dyba; Zbigniew Miszalski; Maria Zembala

doi:10.18388/abp.2020_5230

Elżbieta Rudolphi-Szydło Department of Biochemistry and Biophysics, Institute of Biology, Pedagogical University of Cracow, Kraków, Poland http://orcid.org/0000-0001-6721-3703
Maria Filek Department of Biochemistry and Biophysics, Institute of Biology, Pedagogical University of Cracow, Kraków, Polan https://orcid.org/0000-0001-7259-8353
Barbara Dyba Department of Biochemistry and Biophysics, Institute of Biology, Pedagogical University of Cracow, Kraków, Polan https://orcid.org/0000-0001-5528-5128
Zbigniew Miszalski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland https://orcid.org/0000-0002-2460-1498
Maria Zembala Department of Biochemistry and Biophysics, Institute of Biology, Pedagogical University of Cracow, Kraków, Poland https://orcid.org/0000-0001-8684-6725

DOI: https://doi.org/10.18388/abp.2020_5230

Abstract

In the present work, Langmuir monolayers were used to study the interaction of putrescine (a cationic antioxidant) with anionic charged membranes (1,2-dioleoyl-sn-glycerol-3-phosphate) under oxidative stress caused by the presence of ozone in the water phase. Calcium ions and acidic environment were used to compare the electrostatic and antioxidant effects of putrescine with those of an inorganic cation. It has been shown that the main role of putrescine in protecting systems against oxidation is its rapid reaction with ROS. The initial rate of ROS neutralization rose as the concentration of putrescine increased. No such reaction was observed for calcium ions. The consequence of putrescine’s ozone removal was lesser lipid destruction that depended on the pH conditions.