Recent developments in MALDI MSI application in plant tissues analysis

Katarzyna Susniak; Mikołaj Krysa; Barbara Gieroba; Iwona Komaniecka; Anna Sroka-Bartnicka

doi:10.18388/abp.2020_5394

Katarzyna Susniak Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland; https://orcid.org/0000-0002-1360-5901
Mikołaj Krysa Department of Biopharmacy, Medical University of Lublin, Lublin, Poland https://orcid.org/0000-0001-6853-5528
Barbara Gieroba Department of Biopharmacy, Medical University of Lublin, Lublin, Poland https://orcid.org/0000-0001-8549-9848
Iwona Komaniecka Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland https://orcid.org/0000-0003-0807-8083
Anna Sroka-Bartnicka 1Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland; 2Department of Biopharmacy, Medical University of Lublin, Lublin, Poland https://orcid.org/0000-0003-0739-3567

DOI: https://doi.org/10.18388/abp.2020_5394

Abstract

Mass spectrometry imaging (MSI) combined with matrix-assisted laser desorption/ionization (MALDI) is an efficient technology applied in plant metabolomics research. This technique allows for visualization of spatial distribution of metabolites such as: lipids, proteins, peptides and DNA sequences, by determining the x, y coordinates of the compounds exactly in plant tissue. Simplicity of the tissue preparation without the need of prior exact knowledge about the analytes is a great advantage of this method. In this review, we provide an overview of experimental workflow including sample preparation, data acquisition and analysis, methodology, and some recent applications of MALDI MS imaging in plant metabolomics research.