Abundance, fatty acid composition and saturation index of neutral lipids in colorectal cancer cell lines

  • Rimsha Munir 1Cancer Biology Lab, Institute of Microbiology and Molecular Genetics , University of the Punjab, Lahore, Pakistan; 2Cancer Research Centre (CRC), University of the Punjab, Lahore, Pakistan
  • Jan Lisec Bundesanstalt für Materialforschung und -prüfung (BAM), Berlin, Germany
  • Carsten Jaeger Bundesanstalt für Materialforschung und -prüfung (BAM), Berlin, Germany
  • Nousheen Zaidi 1Cancer Biology Lab, Institute of Microbiology and Molecular Genetics , University of the Punjab, Lahore, Pakistan; 2Cancer Research Centre (CRC), University of the Punjab, Lahore, Pakistan https://orcid.org/0000-0002-3896-0088

Abstract

Lipid droplets, the dynamic organelles that store triglycerides (TG) and cholesterol esters (CE), are highly accumulated in colon cancer cells. This work studies the TG and CE subspecies profile in colon carcinoma cell lines, SW480 derived from primary tumor, and SW620 derived from a metastasis of the same tumor. It was previously reported that the total TG and CE content is dramatically higher in SW620 cells; however, TG and CE subspecies profile has not been investigated in detail. The work presented here confirms that the total TG and CE content is significantly higher in the SW620 cells. Moreover, the fatty acid (FA) composition of TG is significantly altered in the SW620 cells, with significant decrease in the abundance of saturated triglycerides. This resulted in a significantly decreased TG saturation index in the SW620 cells. The saturation index of CE was also significantly decreased in the SW620 cells.

Published
2021-02-17
Section
Articles